Practical sheet | REF: FIC1417 V1

Using micropipettes for testing

Author: Nicolas LEPETIT

Publication date: March 10, 2015 | Lire en français

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    3. How do I check my pipette?

    Metrological verification is carried out using gravimetry, for example.

    Here are a few remarks on the gravimetric method and the standard that deals with the subject.

    3.1 Acceptability criteria

    The standard dealing with gravimetric verification is EN ISO-8655. This standard gives acceptability criteria based on nominal volumes, which is debatable as they are expressed as absolute values. For example, for a 1,000 µl pipette (nominal volume = 1,000 µl), the standard gives an acceptable accuracy error of 8 µl. It may seem reasonable to accept 8 µl of error at 1,000 µl (i.e. 0.8%), but most current 1,000 µl pipettes have a range of use from 100 µl to 1,000 µl, and the standard recommends checking three volumes, one of which should be...

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    How do I check my pipette?