1. General presentation
Either to measure a substance S (a hormone H, for example), in a sample of a given biological medium (serum); the choice of the best assay method to use will have to meet four imperatives:
its detection limit or detectability must be as low as possible; as the concentration of S in the medium is generally very low, in the picomole range (10 –12 mole), the method chosen must have a sufficient detection limit, while remaining reliable (for example, normal values for thyroid hormones: [FT4] 10 to 25 pmol.L –1 ; [FT3] 4 to 8 pmol.L –1 );
it must be specific; a biological medium is always extremely complex, and the technique used must specifically assay substance S alone and...
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General presentation
Antigen-antibody system
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