3. Radioimmunoassays
According to their principle, to achieve a good RIA (competition) or IRMA (sandwich) assay, the reagents and the various reaction steps must meet certain conditions:
the substance, or , must be labelled with an appropriate radio-nuclide (iodine, tritium);
The Ac antibody used must ensure maximum specificity for the reaction, in particular by using monoclonal antibodies;
free and bound fractions must be completely separated, so that only the activity of one or the...
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Radioimmunoassays
Radioactive signal detection
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