3. Columns and stationary phases
SPC uses the vast majority of filled columns used in LC and, very exceptionally, capillary columns used in GC, where the stationary phase is immobilized.
3.1 Filled columns
The same geometries and variety of stationary phases are used in SPC as in LC and UHPLC, including the vast majority of chiral stationary phases (with the exception of stationary phases operating solely with aqueous eluent or at risk of denaturation in organic media, such as protein-grafted phases). However, wherever possible, working conditions (P, T and mobile phase flow rate) should be chosen to minimize pressure drop, which can lead to a density gradient in the column and hence to thermodynamic spreading of the solute band
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Columns and stationary phases
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