4. Artifacts related to experimental conditions and tissue preparation
4.1 Distribution anomaly of radiolabelled compound
Low binding rates and limited biodistribution of the radiolabeled compound can be a source of false-negative images. This problem may result from poor barrier passage, poor blood flow, or impaired active or passive membrane transport (diffusion) due to membrane damage.
4.2 Leakage and redistribution of radiolabelled compound and tissue constituents
Non-covalently bound radiolabeled compounds and tissue constituents not immobilized by fixatives...
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Artifacts related to experimental conditions and tissue preparation
Quantitative autoradiography
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