4. Detection of TBT by the bioluminescent bacterium Escherichia coli TBT3
The idea was to develop a bacterium whose bioluminescence would be enhanced (induction) in the presence of a pollutant such as TBT.
4.1 Construction of the bacteria
Bioluminescence is the natural production of light by living organisms. These organisms include a number of bacterial species, most of them marine. The reaction is catalyzed by the enzyme luciferase. In the presence of oxygen, this enzyme catalyzes the oxidation of a long-chain aldehyde and reduced flavin mononucleotide (FMNH 2 ) to fatty acid and FMN. This reaction is accompanied by the emission of light at 490 nm and 590 nm as shown in figure
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Detection of TBT by the bioluminescent bacterium Escherichia coli TBT3
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