Organic matter fractionation techniques of liquid waste for the modeling of bioprocesses

Biological processes are widely used to treat liquid and solid waste containing a high proportion of biodegradable matter. In order to optimize them, they are often studied using modeling tools, the use of which involves compartmentalizing organic matter into several fractions that are more or less rapidly degradable by bacteria. This fractionation requires the use of specific experimental methods, which are still poorly described and whose differences are not always clearly identified. However, the systematic application of a protocol for fractioning organic matter into biodegradability classes is essential for simulating processes and optimizing bioprocesses.

This article presents the existing concepts and methodologies for fractioning organic matter, and provides feedback and advice on the choice of methods for use by engineers.