3. Formation of pre- or post-chromatography derivatives
When the solute has no chromophore groups suitable for spectrophotometric detection, it must be transformed with a specific reagent.
-
This can be done prior to chromatography
[14] oxidation or reduction using hydrogen peroxide, sodium periodate, chromic acid, iodine or palladium chloride, sodium borohydride, etc. ;
hydrolysis, which may be chemical or enzymatic; zone concentration plates are well suited to the latter technique;
...
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Formation of pre- or post-chromatography derivatives
Calibration curves
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