2. Magnusson and Kligman's Guinea Pig Maximization Test (M&K - OECD test no. 406: skin sensitization)

The method combines intradermal injections and epidermal application. It uses Freund's complete adjuvant and sodium lauryl sulfate to stimulate the sensitization-inducing reaction.

Induction takes place through a number of different processes.

2.1 Intradermal injections

Day 0 – treatment group: three pairs of 0.1 ml intradermal injections are performed in the hair-free shoulder area, so that one injection of each pair is located on each side of the midline of the body:

injection 1: 1:1 (v/v) mixture of Freund's complete adjuvant (ACF) and water or saline ;
injection 2: test substance in a suitable vehicle at the chosen concentration ;

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Magnusson and Kligman's Guinea Pig Maximization Test (M&K - OECD test no. 406: skin sensitization)