3. How are in vitro skin irritation and corrosion tests conducted?
In order to limit the use of laboratory animals, numerous tests have been developed, some of which have been validated by the OECD.
3.1 In vitro" skin irritation
OECD test no. 439: in vitro skin irritation reconstructed human epidermis test method. Principle: "The test substance is applied topically to a three-dimensional model of reconstructed human epidermis, composed of untransformed keratinocytes taken from human epidermis and cultured to form a highly differentiated multilayer model of human epidermis. This model is composed of organized layers (basal, spinous and granular), as well as a multilayered stratum corneum containing intercellular lamellar lipid layers representing the main lipid classes, similar to those observed in vivo. The cell viability...
The Ultimate Scientific and Technical Reference
This article is included in
Chemical risk prevention manager
This offer includes:
Knowledge Base
Updated and enriched with articles validated by our scientific committees
Services
A set of exclusive tools to complement the resources
Practical Path
Operational and didactic, to guarantee the acquisition of transversal skills
Doc & Quiz
Interactive articles with quizzes, for constructive reading
How are in vitro skin irritation and corrosion tests conducted?
What is the impact of a chemical substance on the eye?
Bibliography
A. Lombard et al. – Techno-economic evaluation of products in the early stages of development. Part 2, pp. 153-281. ISTE Éditions, Ltd London (2024).
The Ultimate Scientific and Technical Reference
